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After a week, the cover can be gradually removed and the plants acclimated to stronger light and drier atmospheric conditions. You now have a collection of plants in your classroom that are genetically exactly the same. You could use these plants to carry out other experiments knowing that one common source of variation in the experiment has been eliminated. Some of these tests could include looking at plant responses to low light levels, to drought, or to saline soil conditions.
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Each container can be used to prepare about 30 tubes as above. The first container should have BAP added at the rate of 2.0 mg/l. The second container should have the NAA hormone added at the rate of 0.1 mg/L. To do this, it is necessary to make concentrated solutions of both BAP (2.0 mg/ml) and NAA (1.0 mg/ml). Add 1 ml of the concentrated BAP stock or 100 µl of the NAA concentrated stock to each 1 liter of medium that you prepare. If you use rooting hormone purchased from your local hardware or nursery supply store instead of NAA, then just follow the directions before adding to your medium.
Plant tissue culture involves excising plant tissues and growing them on nutrient media. It is used rather broadly to include several variations, such as meristem culture for propagation of virus-free plants, protoplast culture, cell suspension culture, tissue and organ culture, and anther or pollen culture for producing haploid plants. This chapter focuses on various technical aspects of plant tissue culture. A suitable explant is selected and prepared for culture, and later incubated on an appropriate nutrient medium for growth and differentiation.
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