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Plant tissue culture involves excising plant tissues and growing them on nutrient media. It is used rather broadly to include several variations, such as meristem culture for propagation of virus-free plants, protoplast culture, cell suspension culture, tissue and organ culture, and anther or pollen culture for producing haploid plants. This chapter focuses on various technical aspects of plant tissue culture. A suitable explant is selected and prepared for culture, and later incubated on an appropriate nutrient medium for growth and differentiation.
Each plant should be carefully removed from its tube of medium and planted into a small pot containing a clean light potting mix. Gently wash off all of the agar medium prior to planting. The plants will still need to be protected at this stage since they are not accclimated to the drier air of the classroom when compared to the moist environment of the tube. Place all of the pots onto a tray and cover loosely with a plastic dome or tent. Place the plants in an area with 12-16 hours of light (either natural or artificial) but not direct sunlight.
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Tissue culture involves the use of small pieces of plant tissue (explants) which are cultured in a nutrient medium under sterile conditions. Using the appropriate growing conditions for each explant type, plants can be induced to rapidly produce new shoots, and, with the addition of suitable hormones new roots. These plantlets can also be divided, usually at the shoot stage, to produce large numbers of new plantlets. The new plants can then be placed in soil and grown in the normal manner.
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Foshan Youngplants Co., Ltd has grown into a large biotechnological known as ‘The Rising Star in the Field of Tissue Culture’, with 6 specialized in vitro tissue culture laboratories covering a total area of more than 11,000㎡. After 14 years’ efforts, our company has specialized in supplying in vitro tissue culture plants to both local and worldwide markets, and has eventually increased yearly output to over 80 million with stable quality and rich varieties. With the helps and supports of our global clients, professors and officials, we gained a strong reputation.